article thumbnail

Biochemical assays and deep cyclic inhibition in cancer treatment

Drug Target Review

Molecular-level biochemical assays like transcriptomics, genomics and proteomics have emerged as valuable tools for identifying potential targets in cancer treatment through deep cyclic inhibition (DCI).

article thumbnail

Positive Impact of Dragonfly™ Discovery in Biochemical Assay

Zobio

Biochemical assays are commonly used to assess how organic compounds impact the activity of a protein of interest (POI). Het bericht Positive Impact of Dragonfly™ Discovery in Biochemical Assay verscheen eerst op ZoBio - Drug Discovery Technology.

Insiders

Sign Up for our Newsletter

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

article thumbnail

Biochemical and Structural Studies of Protein Tyrosine Phosphatase PTP‐PEST (PTPN12) in Search of Small Molecule Inhibitors

Chemical Biology and Drug Design

The invitro biochemical assays validated that J1-65 inhibits PTP-PEST activity competitively and the inhibitor binding stabilizes the protein-ligand complex. An in-house molecule library was screened using in silico molecular docking, and a myo-inositol derivative was identified as a potent hit molecule.

article thumbnail

A Guide to DNA Damage Response and Innate Immunity

Drug Target Review

The Transcreener HTS Assay platform accelerates these efforts by providing robust and easy-to-use biochemical assays for key enzymes in the pathways. In this guide, we provide an overview of the DDR and innate immune pathways and describe assay systems for key therapeutic targets.

DNA 104
article thumbnail

Evaluation of a panel of furochromenones as the activator and inhibitor of tyrosinase

Chemical Biology and Drug Design

Using fungal tyrosinase-based in vitro biochemical assay, we obtained one compound ( 3k ) which could inhibit tyrosinase activity, and the other compound ( 4f ) that stimulated tyrosinase activity. In the present study, we investigated these compounds for their potential as antagonists or agonists of tyrosinase.

article thumbnail

The peptide DR3penA exhibits antifibrotic activity in multiple pulmonary fibrosis models induced by particulate and soluble chemical fibrogenic agents [Gastrointestinal, Hepatic, Pulmonary, and Renal]

ASPET

Based on the druggability of DR3penA, we sought to investigate its effects on respirable particulate silicon dioxide (SiO 2 )- and soluble chemical paraquat (PQ)-induced pulmonary fibrosis in this study by using western blot, RT-qPCR, immunofluorescence, H&E and Masson staining, immunohistochemistry and serum biochemical assays.

article thumbnail

Betulinic acid reduces inflammation in rats with sepsis?induced myocardial dysfunction by inhibiting TLR4/MyD88/NF??B signaling pathway

Chemical Biology and Drug Design

Then, cardiac function parameters were assessed using echocardiography, myocardial injury markers were quantified via biochemical assays, and myocardial histopathological injuries were observed through H&E staining.