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2‐Hydroxy‐3‐Methylanthraquinone Suppresses Hepatocellular Carcinoma Progression by Blocking Annexin A5‐Mediated Phosphatidylinositol 3‐Kinase/Protein Kinase B Signaling

Chemical Biology and Drug Design

Quantitative real-time polymerase chain reaction and Western blotting were used to evaluate mRNA and protein expression levels. Network pharmacology analysis was conducted to predict potential targets of HMA in HCC. Cell migration and invasion were assessed using wound healing and transwell assays.

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Scientific workflow for hypothesis testing in drug discovery: Part 2 of 3

Drug Target Review

For instance, a dataset generated to study protein expression in one context might also reveal valuable information about other biological pathways or processes. Additionally, exploratory analyses can be valuable for identifying new biological markers or hypotheses.

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Building Korro Bio: A CEO’s Perspective on Innovation and Risk Management

LifeSciVC

We built our proprietary OPERA platform ( here ), leveraging oligonucleotide guided RNA editing to modulate protein expression. We intended to learn from nature (genetics) and use pharmaceuticals properties to drive patient benefit. The experiment worked and showed the precision of RNA editing.

RNA
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Spirulina’s role in shaping the future of preventative biologics

Drug Target Review

Traditional biologics often involve recombinant protein expression in mammalian cells or microbes like E. As with any novel approach, Lumen faced challenges in navigating regulatory frameworks for spirulina-based therapeutics. coli and yeast, but spirulina presents a new set of considerations.

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The Magic of Fast Feedback Loops

Codon

One such opportunity, for example, is in protein expression. When working with proteins — or designing new ones — scientists must first express them within a cellular “host,” which in turn demands that the proteins be successfully translated and folded into a stable, soluble structure.

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Anti‐Osteoporosis Activity of Lycopene Through ESR1: Network Pharmacology, Molecular Docking, Imaging Technology, and Experimental Validation

Chemical Biology and Drug Design

The osteoblastic differentiation of human marrow-derived mesenchymal stem cells (hBMSCs) was induced and evaluated by ALP staining and activity assay, Alizarin Red S (ARS) staining, and related protein expression analysis. An osteoporotic mouse model was induced by ovariectomy (OVX).

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FDA Issues “Cliffs Notes”-style Guidance on Cell and Gene Therapy; What Questions Did They Answer? (Part 1)

FDA Law Blog: Drug Discovery

Where vector presence is detected, transgene mRNA and/or protein expression levels should also be measured. For cell therapy products, the draft guidance notes that in vivo imaging techniques provide certain advantages. For gene therapy products, the use of quantitative and sensitive assays such as qPCR are recommended.