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Protein Engineering and Parallel Protein Expression-Purification: A Match Made in Heaven

Zobio

In an earlier blog article, we discussed the issue of protein aggregation, how to detect it and how we screen solution conditions to overcome it. Many of the other issues with ill-behaved proteins.

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Remimazolam attenuates inflammation in bronchopneumonia through the inhibition of NLRP3 activity by PDPK1 ubiquitination

Chemical Biology and Drug Design

Remimazolam induced PDPK1 and p-AKT protein expressions, and suppressed NLRP3 protein expression in lung tissue of mice model. In vitro model, Remimazolam also induced PDPK1 and p-AKT protein expressions, and suppressed NLRP3 protein expression. Remimazolam interlinked PDPK1 protein.

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CAR-T therapy could “alter the treatment paradigm” of multiple sclerosis

Drug Discovery World

KYV-101, specifically targets CD19, a protein expressed on the surface of B cells, which is involved in various types of autoimmune diseases. CAR T-cell therapy involves modifying a patient’s T cells to recognise and remove B cells in the patient’s body.

Therapies 312
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Diosgenin inhibits proliferation and migration of ovarian cancer cells and induce apoptosis via upregulation of PTEN

Chemical Biology and Drug Design

OVCAR-3 and SKOV-3 cells were treated with diosgenin, cellular viability was assessed by MTT assay and apoptosis was measured by ELISA and evaluated the protein expression levels of apoptotic markers through western blotting. The protein expression levels of main components of PI3K signaling were evaluated via western blotting.

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Curcumin activates the JNK signaling pathway to promote ferroptosis in colon cancer cells

Chemical Biology and Drug Design

Curcumin modulates the gene and protein expression levels of ferroptosis mediators via JNK signaling. In addition, curcumin modulated the mRNA and protein expression levels of ferroptosis-related proteins including ACSL4, GPx4 and FTH1 and suppression of JNK signaling.

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Matrine exerts an anti?tumor effect via regulating HN1 in triple breast cancer both in vitro and in vivo

Chemical Biology and Drug Design

Western blot analysis was applied to detect the protein expression of apoptosis HN1. The effects of matrine on tumor growth, protein expression of HN1, and apoptosis in vivo were validated by xenograft tumor models and histology. HN1 overexpression perceptibly reversed the above-mentioned additive effect in vitro.

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Discovery of small molecule c?Maf inhibitors using molecular docking?based virtual screening, molecular dynamics simulation, and biological evaluation

Chemical Biology and Drug Design

Sorafenib and glimepiride simultaneously downregulated c-Maf protein expression to induce G1 phase arrest and apoptosis in myeloma cells. Moreover, both compounds simultaneously downregulated c-Maf protein expression to induce G1 phase arrest and apoptosis in myeloma cells.