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Prime Editing: Adding Precision and Flexibility to CRISPR Editing

addgene Blog

But prime editing, a CRISPR technique developed in David Lius lab in 2019, has added considerably more precision and flexibility to the CRISPR editing world. All of this is possible without double-strand breaks (DSBs) or donor DNA templates. Plus, it can combine different types of edits with one another.

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Perfume from Extinct Flowers, Thanks to Ancient DNA and Synthetic Biology

PLOS: DNA Science

“Meet Invisible Woods: a clean, refreshing scent revived from extinct flower DNA ,” beneath an image of “origin flower” Wendlandia angustifolia. • compare the DNA sequence to similar genes in other species. • tweak a lab-made copy of the DNA sequence using clues from other plant species.

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Evolved prime editors are smaller and more efficient for therapeutic applications

Broad Institute

Their new editors are more efficient and specialized than previous versions, and are able to modify DNA in cultured cells and in animals that have been difficult to edit, including in immune system cells and inside the brain. In the new work, they focused on improving the heart of the prime editing system—the reverse transcriptase. “We

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PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

addgene Blog

Prime editing is a versatile genome editing technology that allows precise modifications of DNA (replacements, small insertions, and deletions) without introducing DNA double-strand breaks (Anzalone et al., 2019; Chen & Liu, 2023).

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Researchers engineer in vivo delivery system for prime editing, partially restoring vision in mice

Broad Institute

In the new work published today in Nature Biotechnology , the team adapted engineered virus-like particles (eVLPs) that they had previously designed to carry base editors — another type of precision gene editor that makes single-letter changes in DNA.

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Prime editing efficiently corrects cystic fibrosis mutation in human lung cells

Broad Institute

A three-drug cocktail known as Trikafta has greatly improved patient quality of life since its development in 2019, but can cause cataracts and liver damage and must be taken daily at a cost of about $300,000 per year.

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Gerstner Center for Cancer Diagnostics receives additional commitment from Louis V. Gerstner, Jr., to advance technologies that could transform cancer care

Broad Institute

This commitment to the Gerstner Center for Cancer Diagnostics includes $10 million for current support for the Center and $10 million in endowed funding, adding to the $15 million originally committed by Gerstner and an initial endowment of $10 million from the Eli & Edythe Broad Foundation to establish the Center in 2019.

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