CDD Vault Update (February #2 2025): Advanced Macromolecules - Modified Bioconjugates Support in CDD Vault
Collaborative Drug
FEBRUARY 21, 2025
CDD Update February 2025 #2 New Macromolecule Mode Edit DNA, RNA & Peptides with Bioconjugate Support
Drug Discovery Industry Roundup with Barry Bunin — December 18, 2023
Collaborative Drug
DECEMBER 18, 2023
Discover the impact of 'junk DNA' on cancer, teen obesity treatments, Neanderthal genes on sleep patterns, and Nobel discoveries shaping medicine
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Drug Discovery Industry Roundup with Barry Bunin — July, 22 2024
Collaborative Drug
JULY 22, 2024
DNA Discovery that Could Help Find Pancreatic Cancer Cure. Is Revolutionizing Drug Development. Holy Grail for Weight-Loss Drugs Is Sleep Apnea?
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CRISPR 101: Cytosine and Adenine Base Editors
addgene Blog
FEBRUARY 13, 2025
Early CRISPR applications were often limited by the low editing efficiency of homology-directed repair (HDR) , the pathway for resolving DNA double-strand breaks (DSBs) preferred by researchers. Compared to non-homologous end joining (NHEJ) , HDR occurs at a relatively low frequency, especially in nondividing cells.
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Degrading DNA with Cascade-Cas3
addgene Blog
FEBRUARY 11, 2025
The versatility of CRISPR allows you to play with DNA in a number of ways, from small edits that change single base pairs, to chromosomal inversions and large deletions. Many of these methods rely on Cas9 or a derivative of Cas9, but the ever-expanding repertoire of CRISPR has brought many other Cas effectors to light.
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Plasmids 101: Modular Cloning Applications and Kits
addgene Blog
MAY 14, 2024
Modular cloning is a popular DNA assembly tool used to build single- and multi-gene constructs for a variety of applications. In this blog we will review common uses of MoClo and the kits Addgene offers to kickstart these applications, no matter what your model organism is.
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CRISPR 101: Off-Target Effects
addgene Blog
FEBRUARY 8, 2024
For CRISPR work, this often means DNA cleavage at unanticipated sites. In this blog, we will discuss when off-targets can be an issue in CRISPR, how to predict and minimize them, and ways to control for and quantify them when they can’t be avoided.
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AI-Designed Enzymes
Codon
FEBRUARY 13, 2025
They synthesized DNA encoding each protein variant, expressed the proteins in E. He also writes on a blog focused on the intersection of biology and AI at owlposting.com. Achieving a non-zero turnover rate at all is something that nobody has ever done before with enzymes as complex as these.
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Early Career Researcher Toolbox: Free Online Molecular Biology Tools
addgene Blog
SEPTEMBER 12, 2023
DNA sequence analysis. Primer design. Plasmid mapping. We all have our favorite tools for tackling these particular tasks, but they tend to be scattered about the internet. To help you keep your virtual molecular biology toolbox organized, today’s post features a list of free online molecular biology tools all in one place.
Plasmids 101: Stringent Regulation of Replication
addgene Blog
JUNE 20, 2024
As described in our previous Origin of Replication post, DNA replication is initiated at the ori and may or may not be synchronized with the replication of the host cell's chromosomal DNA. All plasmids rely on their host cell's replication machinery in order to replicate—but not always to the same extent.
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DNA Analysis Finds New Target for Diabetes Drugs
NIH Director's Blog: Drug Development
MARCH 11, 2014
Credit: Jane Ades, National Human Genome Research Institute, NIH Type 2 diabetes (T2D) tends to run in families, and over the last five years the application of genomic technologies has led to discovery of more than 60 specific DNA variants that contribute to risk.
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DNA Barcodes Could Streamline Search for New Drugs to Combat Cancer
NIH Director's Blog: Drug Discovery
MARCH 8, 2016
Instead of the black, printed stripes of the Universal Product Codes (UPCs) that we see on everything from package deliveries to clothing tags, they used short, unique snippets of DNA to label cells. DNA barcoding has already empowered single-cell analysis, including for nerve cells in the brain. PRISM consists of two key components.
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PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs
addgene Blog
JUNE 25, 2024
Prime editing is a versatile genome editing technology that allows precise modifications of DNA (replacements, small insertions, and deletions) without introducing DNA double-strand breaks (Anzalone et al., 2019; Chen & Liu, 2023).
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Build Your CRISPR Vocabulary
addgene Blog
JULY 2, 2024
We’ll cover the common terminology for the proteins, DNA, and RNA used in CRISPR. CRISPR is an elegant bacterial immune system and an efficient gene editing tool… but boy does it have a lot of parts! If you’re still a bit confused by CRISPR acronyms, this post is for you.
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Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)
addgene Blog
SEPTEMBER 26, 2024
coli , SLIC is a cheap, standardized, and rapid multi-part DNA assembly method. Not only does this system not use site-specific recombination, it also doesn’t require a ligation step. Based on the robust system of homologous recombination found in E. Read on to learn how to use it in your research!
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Antibodies 101: Producing Recombinant Antibodies
addgene Blog
AUGUST 13, 2024
Since recombinant antibodies conveniently allow for unlimited production, reliable expression, and easy distribution as DNA (Trimmer, 2020), you may be interested in using them in your own experiments. It is possible to produce your own recombinant antibodies with some molecular biology and cell culture experience.
Next Generation Sequencing (NGS): why is quantifying nucleic acids important? | BMG LABTECH
BMG Labtech
OCTOBER 4, 2023
These numbers reflect impressive developments in DNA sequencing technologies but in most cases represent the immediate costs of consumables on the sequencing instrument. 2 DNA sequencing is also reliant on sample processing and DNA library preparation before sequencing as well as bioinformatics and data analysis after sequencing.
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Traditional Meat Industry’s Beef With Alternative Protein Continues with the FAIR on Labels Act
FDA Law Blog: Biosimilars
MARCH 5, 2024
Gaulkin & Riëtte van Laack — As readers of this blog know, there is a lot of contention about the naming of alternative protein products (APPs), including both plant-based and cell-cultured alternatives for (traditional) animal products. We’ve previously blogged about this ongoing battle here , here , here , and here. By Sophia R.
Viral Vectors 101: An Introduction to AAV
addgene Blog
MAY 30, 2023
Adeno-associated virus (AAV) is a single stranded, Parvoviridae DNA virus, packaged in a non-enveloped icosahedral capsid, that can be used to express genes of interest in cell and animal models. This post was contributed by guest blogger Didem Goz Ayturk with edits and updates from Addgenies Karen Guerin and Susanna Stroik.
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FDA Eliminates Animal Testing: Impact on Biotechs
The Premier Consulting Blog
APRIL 13, 2025
6] ICH Harmonised Guideline M7(R2) (2023): Assessment and control of DNA reactive (mutagenic) impurities in pharmaceuticals to limit potential carcinogenic risk. link] [7] H.R.2565 2565 – 117th Congress (2021-2022): FDA Modernization Act of 2021 | Congress.gov | Library of Congress. [8]
Novel Therapeutic Candidate Puts the STING Back into Immunotherapy
PerkinElmer
MAY 6, 2022
For example, the STimulator of InterferoN Genes (STING) pathway, which is known to play a critical role in innate immunity in response to viral and bacterial DNA, has also been shown to promote anti-tumor immune responses. link] The post Novel Therapeutic Candidate Puts the STING Back into Immunotherapy first appeared on PerkinElmer Blog.
Analytical Testing Considerations for Gene Therapy Products
The Premier Consulting Blog
MARCH 7, 2024
Depending on the gene therapy vector, microbial systems may be employed to produce vector DNA (such as in adeno associated virus (AAV)), in which case there may be bacterial cell banks and plasmid banks. A more rigorous identity test may sometimes be required, such as DNA sequencing of the entire AAV genome.
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Breaking Down Biomarker Discovery: 5 Points
PerkinElmer
AUGUST 12, 2020
The steps taken during the analytical phase are dependent on the type of biomarker, which can range from DNA, RNA, protein, peptide, biomolecular modification(s), or biochemical pathway(s). The post Breaking Down Biomarker Discovery: 5 Points first appeared on PerkinElmer Blog.
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Evaluating Impurities in New Drugs to Prevent Delays in Development
The Premier Consulting Blog
JULY 9, 2023
Assessment and control of DNA reactive impurities Another important guidance document related to impurities and degradants in new small molecule drugs is ICH M7(R1). In addition, this guidance focuses on DNA reactive substances that could potentially cause cancer. link] ICH M7 (R2)- Q&A.
Announcing the first biopharma IPNFT Transaction
Molecule Blog
AUGUST 13, 2021
His work focused on the cross-talk between DNA repair and mitochondrial function in aging and has been honored by a number of competitive awards. Announcing the first biopharma IPNFT Transaction was originally published in Molecule Blog on Medium, where people are continuing the conversation by highlighting and responding to this story.
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A Molecular Biologist’s Advice For Life
LifeSciVC
JULY 1, 2024
The central dogma of molecular biology is that information generally (with few exceptions) flows from DNA to RNA to Protein. In my own career, starting to write a blog was one of my most impactful “Friday afternoon experiments” – I even called it an experiment in my very first blogpost back in March 2011. Remember life’s Central Dogma.
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Code breaking and the Human Genome Project
The Open Targets Blog
APRIL 25, 2023
via Wikimedia Commons) Right: Copy of a DNA sequence faxed in 1989 from teams working on the Human Genome Project (from the Wellcome Collection archives, Image credit: Helena Cornu) Assembling a team At the core of this of course were people to provide both the intellectual input and the perseverance to make things happen.
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Missing Genes Point to Possible Drug Targets
NIH Director's Blog: Drug Development
APRIL 18, 2017
Every person’s genetic blueprint, or genome, is unique because of variations that occasionally occur in our DNA sequences. Most of those are passed on to us from our parents. But not all variations are inherited—each of us carries 60 to 100 “new mutations” that happened for the first time in us. Saleheen D et al. 2017 April 12 544: 235-239.
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Top Ten Reasons to Attend the SLAS International Conference & Exhibition
PerkinElmer
JANUARY 19, 2020
We will present a fully automated end-to-end solution inclusive of automation and reagents optimized for miniaturized NGS that is guaranteed to generate high-quality libraries.
What Took So Long: How Tech Has Finally Caught Up With Transcription Factors
LifeSciVC
DECEMBER 6, 2022
The following blog post provides some valuable real-world perspective on why this initiative is such an important breakthrough in immunology therapeutics. These hair clip-like molecules clamp onto DNA, thereby turning many genes on and off.
A Look Back
Dark Matter Blog
JULY 16, 2021
As soon as I learned about DNA and RNA, I wanted to be a molecular biologist. Last stops at RNA My last roles in biotech were where my original passion began: DNA and RNA. We saw a new phenomenon with the emergence of social media that enabled us to follow the experiences of patients through their blogs and social media postings.
South Korea Aims to Establish Biohealth Industry as Strategic Sector, Following Success in Semiconductor and Biopharmaceutical Production
FDA Law Blog: Biosimilars
APRIL 4, 2023
Innovative medical devices; Innovative and essential pharmaceuticals; Digital health care; Advanced regenerative medicine and advanced bio-pharmaceuticals; DNA tests; Brain-machine interface; Infrastructure” We are excited to hear that South Korea is investing in the growth of the biohealth industry and promoting digital healthcare.
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Greater Than The Sum Of The Parts: A Framework For Leading Enterprises In Non-Traditional Ways
LifeSciVC
FEBRUARY 16, 2024
Choosing the wrong candidate from an artificially limited pool is ultimately disruptive and risks imprinting the wrong habits in the company’s DNA. How Boards can best support such a candidate for an extended period when they’re not in the succession plan for CEO could be the subject of another blog post.
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Missing Metabolites
Metabolite Tales Blog
OCTOBER 5, 2023
Reasons for these “missing” metabolites are described in this paper , along with real case studies and advice on how to best to “expect the unexpected” Reasons for missing metabolites comprise: Metabolites invisible in LC-MS Metabolites with reduced ionization in mass spectrometry Biotransformation mediated loss of radiolabel (..)
From hibernation to neurodegeneration: how a cold-resistance protein is paving the way to dementia treatments
The Open Targets Blog
AUGUST 15, 2023
Poison exons are part of a larger group of hidden or cryptic exons, so-called because these sequences are hidden among the gene’s introns, the sections of DNA that don’t encode a protein sequence but that are important in the regulation of the gene’s expression. The mini-gene experiment was a tricky one.
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Reinventing the small molecule toolbox: from proteins to RNA
Dark Matter Blog
APRIL 4, 2023
We sampled several screening platforms in the course of our efforts, including fragment screening using ligand-observed nuclear magnetic resonance (NMR), DNA-encoded libraries (DEL), and SEC-MS. And unproductive interactions between the RNA target and the DNA barcodes complicated DEL screening.
Reaching cruising altitude: New discovery tools to target RNA
Dark Matter Blog
AUGUST 31, 2020
We stuck with our trusty aptamer system, but instead of running the DNA products made by RT on a gel, we attached them to adapters that enabled next-generation sequencing. With millions of DNA sequences, we could precisely measure the rate of RT pausing at each position of the RNA.
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Open Targets Genetics: version 8 is out!
The Open Targets Blog
OCTOBER 13, 2022
The DNA in a gene is made up of exons (the parts that can be used to make proteins) and introns (the parts that will never be transcribed). The existing analyses for QTLs, including credible sets, and GWAS-Molecular Trait colocalisation, are now available for sQTLs.
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How the Open Targets Platform Integrates Gene Burden Analyses
The Open Targets Blog
MARCH 6, 2023
release of the Open Targets Platform integrates new gene burden analyses, structural variants from ClinVar, DailyMed drug label text-mining evidence, an NLP classification of why clinical trials stopped, as well as new data from the Gene2Phenotype cardiac panel Open Targets Blog Sir Target The addition of gene burden data was a major part of our 22.04
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Synthetic biology, the answer to ageing, and more at Research & Innovation 2023
Elrig
FEBRUARY 23, 2023
In this blog, we give you the answer. Read our recent blog to find out why you shouldn’t hesitate to put together your application, right now! That’s why we created the Research & Innovation (R&I) Conference over 5 years ago, now one of ELRIG’s renowned flagship events. But what is Research & Innovation?
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Five Promising Treatment Areas in Early-Phase Drug Development in 2024
Alta Sciences
APRIL 17, 2024
In this blog article, we review some of these areas of investigation where Altasciences has robust expertise and solution offerings. Several viral vectors are being used currently, in addition to non-viral vectors, such as oligonucleotides, naked DNA, and lipoplexes and polyplexes.
The Year In Review: 2023
KIF1A
MAY 31, 2024
You can read about her journey and first dose in our blog. KAND Cell and Mouse Models, and Prime Editing with the Jackson Laboratory The Jackson Laboratory, which has developed 3 KAND mouse models and 9 KAND cell models, is working on prime/base editing, which precisely replaces sections of mutant KIF1A DNA with the healthy sequence.
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TACACICLIB, AUR-102, AURIGENE
New Drug Approvals
JANUARY 9, 2024
CDK7 also plays a role in transcription and possibly in DNA repair. The trimeric Cak complex CDK7/CyclinH/MATl is also a component of TFIIH, the general transcription/DNA repair factor IIH (Morgan, DO., Cyclin-dependent kinase 7 (CDK7) activates cell cycle CDKs and is a member of the general Transcription factor II Human (TFIIH).
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The Love Songs of W. E. B. Du Bois Celebrates Afro-Indigenous History with Genealogy – No DNA Needed
PLOS: DNA Science
MAY 18, 2023
Perhaps more telling, I recently discovered a previously unknown branch of my own family tree that includes a dozen half-siblings that I know of, from a DNA test that I didn’t even remember taking. I told my story in a New York Times Modern Love podcast and in blog posts and articles. Which one was our biodad?
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