addgene Blog

OCTOBER 19, 2023

Adeno-associated viruses (AAV) are an important biological tool that allow scientists to express a gene of interest in a cell or animal model. AAV plasmids contain several important elements, but among the most important are the inverted terminal repeats (ITRs). ITRs are the elements that make an AAV plasmid an AAV plasmid.

Virus 124

Virus 124

addgene Blog

OCTOBER 3, 2023

Once ~400 cases had amassed in places around the world, hypotheses began to develop that this illness was caused by either SARS-CoV-2, Adenovirus type 41 (AdV-41), or Adenovirus-Associated Virus type 2 (AAV2) – or some unprecedented combination of the three (Servellita et al.,

Virus 126

Virus 126

addgene Blog

JULY 25, 2023

So, if you’ve ever wondered what goes into ‘making virus’ or what gag and pol stand for, we demystify all components of viral vectors here! For safety and production reasons, multiple plasmids with many components are used to package lab grown viruses. It can be intimidating at first to look at so many plasmid maps and protocols!

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Virus Packaging Production 98

addgene Blog

JULY 30, 2024

As editor of Addgene’s eBooks — including the recently released Viral Vectors 101 2 nd edition — I was incredibly excited when we picked our viral vector service as one of those stories.

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Virus Engineering 98

addgene Blog

JUNE 1, 2023

While it is tempting to just dive right in and start injecting your virus, a successful AAV experiment starts with validation and optimization. Using AAV vectors in complex biological systems can be tricky at best, and downright infuriating at worst.

Virus 98

Virus 98

addgene Blog

AUGUST 15, 2024

What do a viral vector production facility, food allergy testing lab, and the grad student down the hall from you have in common? All of them rely on standard curves in their day-to-day work.

Virus 98

Virus Production 98

NIOSH Science Blog: Drugs

FEBRUARY 3, 2023

Air filtration units were widely used during large wildfire outbreaks in the western United States,[1] and their use surged nationwide during the COVID-19 pandemic as a way to reduce exposure to aerosols containing the SARS-CoV-2 virus.

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Virus Production Engineering Disease 97

PerkinElmer

MAY 28, 2020

Unfortunately, the road towards treatment can be time-consuming, especially when utilizing assays with live virus. Additionally, procuring the live virus for an evaluation assay is very difficult and labor intensive. Such high safety measures, although essential, can extend the timing of the development of a vaccine.

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Vaccine Disease Virus Small Molecule 52

PerkinElmer

JULY 13, 2020

The complex relationship between a virus and its host is one of the hottest topics in science right now, and has been given considerable attention in recent weeks as a result of the race to understand the virus that causes COVID-19 and its potential vulnerabilities.

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RNA Virus Laboratories Science 52

NIOSH Science Blog: Drugs

FEBRUARY 8, 2023

Avian influenza A viruses are classified into the following two categories: Highly pathogenic avian influenza (HPAI) virus strains are extremely infectious, often fatal to chickens, and can spread rapidly from flock-to-flock. Avian influenza (bird flu) is caused by infection with avian influenza (flu) Type A viruses.

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Virus Disease 61

addgene Blog

MAY 30, 2023

Adeno-associated virus (AAV) is a single stranded, Parvoviridae DNA virus, packaged in a non-enveloped icosahedral capsid, that can be used to express genes of interest in cell and animal models. This post was contributed by guest blogger Didem Goz Ayturk with edits and updates from Addgenies Karen Guerin and Susanna Stroik.

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Virus Packaging DNA Laboratories 67

Nvidia Developer: Drug Discovery

APRIL 20, 2020

To help make the process for scientists more accessible, we’re spotlighting a few of the GPU-accelerated applications that developers can use right now in the fight against this virus. Those compounds are likely to bind to the glycosylated spike (S) protein, which is how the virus enters host cells.

Small Molecule 52

Small Molecule Virus Laboratories Science 52

NIH Director's Blog: Drug Discovery

NOVEMBER 5, 2020

Because SARS-CoV-2 is so highly infectious, basic researchers without access to specially designed biosafety facilities may be limited in their ability to study the virus. While the quantum dots may bind and enter human cells just like the virus, they can’t cause an infection. But these harmless quantum dots offer a safe workaround.

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Research Laboratories Laboratories Science Drugs 85

Advarra

AUGUST 8, 2023

In this blog, we summarize the growth, risks, and regulatory requirements for gene therapy research. Viral infection involves the transfer of the virus’ genetic material to host cells, making viruses ideal tools for gene transfer—once the genes responsible for viral replication and disease are removed.

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Therapies Research Engineering Clinical Research 52

The Premier Consulting Blog

MARCH 7, 2024

For gene therapy vectors, this testing encompasses production cell lines, master and working cell banks (MCB, WCB), and virus banks. Depending on the gene therapy vector, microbial systems may be employed to produce vector DNA (such as in adeno associated virus (AAV)), in which case there may be bacterial cell banks and plasmid banks.

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Production Therapies DNA Virus 52

FDA Law Blog: Biosimilars

OCTOBER 24, 2023

To learn more about our experience with the eSTAR for 510(k)s, check out our previous blogs ( here , here ). Note, however, that there is a virus scanning and technical screening process that will still happen. Make sure you submit your application using the most up-to-date version of eSTAR, as FDA frequently updates eSTAR templates.

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FDA Virus Regulations Production 52

Alta Sciences

JANUARY 11, 2024

There are three primary vectors employed in gene therapy: adeno-associated virus (AAV), adenovirus, or lentivirus vectors. Image Blog-Thumbnail_The-Altascientist-issue-36.jpg Some therapies are considered both cell and gene therapies, as they alter genes in specific types of cells.

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Therapies Immune Response Disease Virus 52

FDA Law Blog: Drug Discovery

DECEMBER 7, 2022

At the same time, it is understandably important to be able to measure and characterize a treatment’s effect over time. However, with this most recent approval, FDA did not leave the question of durability as something to be answered postapproval, which signals to us that this issue looms large in FDA’s preapproval regulation of gene therapies.

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Therapies FDA FDA Approval Treatment 119

NIH Director's Blog: Drug Development

OCTOBER 27, 2020

Another promising strategy is drugs that target the proteins within human cells that the virus needs to infect, multiply, and spread. These studies narrowed the list to 73 human proteins that the virus depends on to replicate. That’s the strategy employed by remdesivir , the only antiviral drug currently authorized by the U.S.

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Drug Development Drugs Virus Science 52

Common Sense for Drug Policy Blog

JULY 22, 2023

A syndemic of opioid overdose, human immunodeficiency virus (HIV), hepatitis C virus (HCV), skin and soft tissue infections (SSTI), and infective endocarditis accounts for many of the poor health outcomes among PWUH [ 2 , 3 , 4 , 5 ]. Heroin can be consumed in several ways, including injection and smoking [ 6 ]. McMahan, V.M.,

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Virus Drugs 40

New Drug Approvals

JANUARY 31, 2024

BLZ945 decreases the growth of malignant cells in the mouse mammary tumor virus-driven polyomavirus middle T antigen (MMTV-PyMT) model of mammary carcinogenesis. BLZ945 attenuates the turnover rate of TAMs while increasing the number of CD8+ T cells that infiltrate cervical and breast carcinomas. ES/MS m/z 398.1(MH 3 : 100091.

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Small Molecule Virus Treatment Disease 52

The Pharma Data

DECEMBER 20, 2020

Services will include the production and publication of investor bulletins, distribution of investor bulletins to the Consultant’s e-mail list, and posts via the Consultant’s blogs and social media accounts. In consideration of these services, the Company has paid the Consultant a fee of US$50,000 for a 6-month contract.

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Production Clinical Trials Licensing Licensing 52

NIH Director's Blog: Drug Development

APRIL 2, 2020

ACE2 is the receptor that the virus uses to gain entry. It’s a paper published in the journal Science [1] that presents an atomic-scale snapshot showing the 3D structure of the spike protein on the novel coronavirus attached to a human cell surface protein called ACE2, or angiotensin converting enzyme 2.

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Drug Development Drugs Science Medical Schools 52

Common Sense for Drug Policy Blog

OCTOBER 30, 2023

viral transmission of Human immunodeficiency virus (HIV) and Hepatitis C (HCV) through shared drug use equipment, fatal and nonfatal overdose), rather than exclusively targeting drug consumption itself [ 3 , 4 , 5 , 6 , 7 ]." Thus, practical strategies are necessary to reduce health-related harms associated with drug use (e.g.,

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Virus Treatment Drugs 40

Metabolite Tales Blog

JULY 5, 2023

[link] [6] PHARMACOLOGY/TOXICOLOGY NDA REVIEW AND EVALUATION Study NC219 [link] [7] Acute Liver Injury due to Glecaprevir/Pibrentasvir in a Patient with Chronic Hepatitis C Virus Infection without Cirrhosis. link] Take a look at our other blogs The post Metabolism of macrocyclic drugs appeared first on Hypha Discovery.

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Drugs Metabolic Stability FDA Approval Treatment 52

Metabolite Tales Blog

JULY 5, 2023

[link] [6] PHARMACOLOGY/TOXICOLOGY NDA REVIEW AND EVALUATION Study NC219 [link] [7] Acute Liver Injury due to Glecaprevir/Pibrentasvir in a Patient with Chronic Hepatitis C Virus Infection without Cirrhosis. link] Take a look at our other blogs The post Metabolism of de novo designed macrocyclic drugs appeared first on Hypha Discovery.

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Metabolic Stability Drugs FDA Approval Treatment 52

Common Sense for Drug Policy Blog

JANUARY 1, 2024

18 The average cost of a hepatitis A–related hospitalization in 2016 was $16 610, and recent hepatitis A virus outbreaks alone have cost the nation at least $270 million since 2016. The estimated cost of providing health care services to persons living with chronic HCV infection is $15 billion annually.

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Virus Government Hospitals Treatment 40

New Drug Approvals

APRIL 18, 2023

Approximately 76% of HIV-patients with measurable viral load are infected with a strain of virus that is resistant to one or more classes of antiretroviral agents, thus reducing treatment options. AMD-070 targets the CXCR4 receptor on HIV and prevents the virus from entering and infecting healthy cells.

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Clinical Trials Virus Small Molecule Trials 52

Elrig

FEBRUARY 23, 2023

In this blog, we give you the answer. Read our recent blog to find out why you shouldn’t hesitate to put together your application, right now! That’s why we created the Research & Innovation (R&I) Conference over 5 years ago, now one of ELRIG’s renowned flagship events. But what is Research & Innovation?

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Research Disease Science Drugs 40

NIH Director's Blog: Drug Discovery

MARCH 8, 2016

Golub and colleagues produced the library by using a virus to insert a distinct DNA barcode, just 24 nucleotides long, stably into the genomes of each genetically distinct tumor cell line. This innovative research was led by the lab of Todd Golub of the Broad Institute of MIT and Harvard, Cambridge, MA. PRISM consists of two key components.

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DNA Drugs Small Molecule FDA Approval 52

FDA Law Blog: Biosimilars

JUNE 22, 2023

HbA1c, diagnosis of a particular virus, etc.). Therefore, instead of a general tool-type indication for collection of a sample matrix (e.g., saliva, capillary blood), separate clearances are often required for each application (e.g., As a thought experiment, consider what the state of the U.S.

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FDA Laboratories Regulations Doctors 59

New Drug Approvals

JANUARY 31, 2024

Individuals with 2 APOL1 risk alleles are at increased risk of developing primary (idiopathic) FSGS, human immunodeficiency virus (HIV)-associated FSGS, and NDKD. Human genetics support a causal role for the G1 and G2 APOL1 variants in inducing kidney disease. The slurry was cooled to 5° C. and allowed to stir. 19 (8): 479–480.

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International Disease Production Small Molecule 52

Collaborative Drug

JANUARY 18, 2024

Epstein Bar Virus and Mutiple Sclerosis and more. Nanoplastics in that bottle of water. Weight-Loss drugs from Eli Lilly.

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Virus Drugs 263

PLOS: DNA Science

JUNE 13, 2024

The virus SARS-CoV-2 emerged in Wuhan, home of a premiere research institute that focuses on viruses. An altered virus could have escaped. Evidence that the virus emerged, or escaped, from the wildlife trade is lacking. Her points: 1. Coincidence? I’ve never thought so. RaTG13 shares about 96.1%

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Virus RNA DNA Science 52

PerkinElmer

APRIL 21, 2020

Although the novel coronavirus is the subject of nearly every media story right now, several years ago it was the Zika virus (ZIKV) gathering a great deal of attention. Here, we describe the novel use of high content imaging solutions (HCS) in a study to identify possible Zika virus inhibitors that could be translated into therapeutic form.

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Virus FDA Approval Laboratories Vaccine 52

New Drug Approvals

DECEMBER 26, 2022

1 heavy chain (1-456) (human vh (homo sapiens ighv1-69*01(ighd)-ighj4*01 (90.1%)) (8.8.19) (1-126) -homo sapiens ighg1*03 Immunoglobulin g1, anti-(human respiratory syncytial virus fusion protein)(human monoclonal med18897.gamma.1-chain), 2] [3] Nirsevimab is designed to bind to the fusion protein on the surface of the RSV virus. [4]

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Virus Clinical Trials Trials Disease 52

PerkinElmer

AUGUST 13, 2020

The virus, which can cause a severe form of pneumonia and lead to acute respiratory distress, currently has no FDA-approved targeted therapeutic or vaccine. For this reason, researchers around the globe are scrambling to understand this novel virus and figure out its potentially targetable vulnerabilities.

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Virus Cell Based Assays FDA Approval Clinical Trials 52