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2‐Hydroxy‐3‐Methylanthraquinone Suppresses Hepatocellular Carcinoma Progression by Blocking Annexin A5‐Mediated Phosphatidylinositol 3‐Kinase/Protein Kinase B Signaling

Chemical Biology and Drug Design

Quantitative real-time polymerase chain reaction and Western blotting were used to evaluate mRNA and protein expression levels. Network pharmacology analysis was conducted to predict potential targets of HMA in HCC. Cell migration and invasion were assessed using wound healing and transwell assays.

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Scientific workflow for hypothesis testing in drug discovery: Part 2 of 3

Drug Target Review

In the next article, we will discuss the tools scientists can use to execute the workflow described in Figure 1. For instance, a dataset generated to study protein expression in one context might also reveal valuable information about other biological pathways or processes. Figure 1: High-level workflow for early drug discovery.

Drugs 69
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Spirulina’s role in shaping the future of preventative biologics

Drug Target Review

Traditional biologics often involve recombinant protein expression in mammalian cells or microbes like E. As with any novel approach, Lumen faced challenges in navigating regulatory frameworks for spirulina-based therapeutics. coli and yeast, but spirulina presents a new set of considerations.

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The Magic of Fast Feedback Loops

Codon

One such opportunity, for example, is in protein expression. When working with proteins — or designing new ones — scientists must first express them within a cellular “host,” which in turn demands that the proteins be successfully translated and folded into a stable, soluble structure.

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Anti‐Osteoporosis Activity of Lycopene Through ESR1: Network Pharmacology, Molecular Docking, Imaging Technology, and Experimental Validation

Chemical Biology and Drug Design

The osteoblastic differentiation of human marrow-derived mesenchymal stem cells (hBMSCs) was induced and evaluated by ALP staining and activity assay, Alizarin Red S (ARS) staining, and related protein expression analysis. An osteoporotic mouse model was induced by ovariectomy (OVX).

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Remimazolam attenuates inflammation in bronchopneumonia through the inhibition of NLRP3 activity by PDPK1 ubiquitination

Chemical Biology and Drug Design

Remimazolam induced PDPK1 and p-AKT protein expressions, and suppressed NLRP3 protein expression in lung tissue of mice model. In vitro model, Remimazolam also induced PDPK1 and p-AKT protein expressions, and suppressed NLRP3 protein expression. Remimazolam interlinked PDPK1 protein.

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Protein Engineering and Parallel Protein Expression-Purification: A Match Made in Heaven

Zobio

Since nearly all projects begin with protein, the GIGO principle means that we must have well-behaved proteins so that all downstream activities such as assays and structural biology do not result in “Garbage Out”. Many of the other issues with ill-behaved proteins.